Xpo4 (NM_020506) Mouse Recombinant Protein

CAT#: TP520257

Purified recombinant protein of Mouse exportin 4 (Xpo4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR220257 representing NM_020506
  Red=Cloning site Green=Tags(s)
  
  MMAAALGPPEVIAQLENAAKVLMAPPSMVSNEQRQHAEHIFLSFRKSKSPFAVCRHILETSKVDYVLFQA
  ATAIMEAVVREWVLLEKGSIESLRTFLLTYVLQRPNLQKYVREQILLAVAVIVKRGSLDKSIDCKSIFHE
  VSQLISSGNPTVQTLACSILTALLSEFSSSSKTSNIGLSMEFHGNCKRVFQEEDLRQIFMLTVGVLQEFS
  RRENLSAQMSSVFQRYLALANQVLSWNFLPPNLGRHYIAMFESSQNVLLKPTESWREALLDSRVMELFFT
  VHRKIREDSDMAQDSLQCLAQLASLHGPIFPDEGSQVDYLAHFIEGLLNTINGIEIEDSEAVGISSIISN
  LITVFPRNVLTAIPSELFSSFVNCLTHLTCSFGRSAALEEVLDKDDMVYMEAYDKLLESWLTLVRDDKHF
  HKGFFTQHAVQVFNSYIQCHLAAPDGTRNLTANGVASREEEEISELQEDDRDQFSDQLASVGMLGRIAAE
  HCMPLLTSLLEERVTRLHGQLQRHQQQFLASPGSSTIDNKMLDDLYEDIHWLILVTGYLLADDTQGETPL
  IPPEIMEYSIKHSSEVDINTTLQILGSPGEKASSIPGYSRTDSVIRLLSAVLRVSEVESRAIRADLTHLL
  SPQMGKDIVWFLKRWAKTYLLVDEKLYDQISLPLSTAFGADTEGSQWIIGYLLQKVISNLSVWSSEQDLA
  NDTVQLLVTLVERRERANLVIQCENWWNLAKQFASRSPPLNFLSSPVQRTLMKALVLGGFAHMDTETKQQ
  YWTEVLQPLQQRFLRVINQENFQQMCQQEEVKQEITATLEALCGIAEATQIDNVAILFNFLMDFLNNCIG
  LMEVYKNTPETVNLIIEVFVEVAHKQICYLGESKAMHLYEACLTLLQVYSKNNLGRQRIDVTAEEEQYQD
  LLLIMELLTNLLSKEFIDFSDTDEVFRGHEPGQAAGRSVSAADVVLYGVNLILPLMSQDLLKFPTLCNQY
  YKLITFICEIFPEKIPQLPEDLFKSLMYSLELGMTSMSSEVCQLCLEALTPLAEQCAKAQETDSPLFLAT
  RHFLKLVFDMLVLQKHNTEMTTAAGEAFYTLVCLHQAEYSELVETLLSSQQDPVIYQRLADAFNKLTASS
  TPPALDRKQKMAFLKSLEEFMANVGGLLCVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 130 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_065252
• Locus ID: 57258
• UniProt ID: Q9ESJ0, A0A0R4J254
• Cytogenetics: 14 C3
• Refseq Size: 3456
• Refseq ORF: 3453
• Synonyms: B430309A01Rik; mKIAA1721
• Summary: Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity).[UniProtKB/Swiss-Prot Function]