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Tut1 (NM_197993) Mouse Recombinant Protein

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SKU
TP511013
Purified recombinant protein of Mouse terminal uridylyl transferase 1, U6 snRNA-specific (Tut1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR211013 protein sequence
Red=Cloning site Green=Tags(s)

MAAVDSDVVSLPRGRFRCCLCDVTTANRPSLDAHLKGRKHRDLVQLRATRKAQGLRSVFVSGFPRDVGSA
QLSEYFQTFGPVANIVMDKDKGVFAIVEMGDISAREAVLSQPKHSLGGHGLRVRPREQKEFQSPASKSPK
GVDSSSHQLVQALAEAADVGAQMVKLVELRELSEAERQLRNLVVALMQEVFTEFFPGCVVHPFGSTVNSF
DVHGCDLDLFLDMGDMEETEPDPKAPKVPETSSLDSALASSLDPQALACTPASPLDSLSPTSVQESESLD
FDTPSSLAPQTPDSALGSDTVTSPQSLPPVSPLQEDRKEGKQGKELELAEEASKDEKEEAAAVLELVGSI
LRGCVPGVYRVQTVPSARRPVVKFCHRPSGLHGDVSLSNRLALYNSRFLNLCSEMDGRVRPLVYTLRCWA
QHNGLSGGGPLLNNYALTLLVIYFLQTRDPPVLPTVAQLTQRAGEGEQVEVDGWDCSFPKDASRLEPSTN
VEPLSSLLAQFFSCVSCLDLSGSLLSLREGRPLMVAEGLPSDLWEGLRLGPMNLQDPFDLSHNVAANVTG
RVAKRLQSCCGAAASYCRSLQYQQRSSRGRDWGLLPLLQPSSPSSLLSAKLIPLPSAPFPQVIMALVDVL
REALGCHIEQGTKRRRSEGARIKDSPLGGVNKRQRLGGQEKSFEEGKEEPQGCAGDHSENEVEEMVIEVR
ETPQDWALLHSGPPEEELPLMTANCLDKAAEHNPMKPEVAGEGSQGETGKEASHPSSVSWRCALWHQVWQ
GRRRARRRLQQQTKEEGRGGPTTGAEWLAMEARVTQELKGPNSEQERPPGEPLLSFVASASQAEQTLTVA
PLQDSQGLFPGLHHFLQGFIPQALKNLLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 94.6 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_932110
Locus ID 70044
UniProt ID Q8R3F9
Cytogenetics 19 A
RefSeq Size 2764
RefSeq ORF 2607
Synonyms 2700038E08Rik; PAPD2; Rbm21; star-PAP; TUTase6; U6-TUTase
Summary Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3' UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3' UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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