Anapc5 (NM_001042491) Mouse Recombinant Protein

CAT#: TP510311

Purified recombinant protein of Mouse anaphase-promoting complex subunit 5 (Anapc5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210311 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMTNGVVHANLFGIKDWVTPYKIAVLVLLNEMGRTGEGAVSLVERRKLNQLLLPLLQGPDITLSKLYKLI
  EESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVF
  KLYTALQQYFQNGEKKTVEDADMDREDGEKQMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQAALLKN
  DETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAEGKSNGEEG
  YGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRADSYVLLEHSV
  KKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSM
  NSLESLNAGVQQNNTESFAVALCHLAELHAEQGCFAAAGEVLKHLKDRFPPNSQHAQLWMLCDQKIQFDR
  AMNDGKFHLADSLVTGITALNGIEGVYRKAVVLQAQNQMTEAHKLLQKLLTYCQKLKNTEMVISVLLSVA
  ELYWRSSSPTIAMPVLLEALALSKEYRLQYLASETVLNLAYAQLILGIPEQALTLLHMAIEPILADGAIL
  DKGRAMFLVSKCQVASAASYDPVKKAEALEAAVQNLSEAKNYFAQVDCRERIRDVAYFQARLYHALGKTQ
  ERNHCAMIFRQLHQELPAHGVPLINHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 81.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001035956
• Locus ID: 59008
• UniProt ID: Q8BTZ4, Q3UFC2
• Cytogenetics: 5 F
• Refseq Size: 2751
• Refseq ORF: 2184
• Synonyms: 2510006G12Rik; AA408751; AA536819; AA986414; Anpc5; APC5
• Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]