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Spire2 (NM_172287) Mouse Recombinant Protein

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SKU
TP510260
Purified recombinant protein of Mouse spire type actin nucleation factor 2 (Spire2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Size:
20 ug
  $1,020.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>MR210260 protein sequence
Red=Cloning site Green=Tags(s)

MARAGGGGAAAPERAGGAARPEPWELSLEEVLKVYEQPINEEQAWAVCFQGCRGLRGEPGGVRRIRDTAD
ILLRRDGSVGARLEPEPTTMVVPPASSEAQMVQSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSD
CEDSSCGAADEGYVGPEEEEEAEGGPRAVRTFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAF
LARVREAKEMLKKLGEEEPREKPLAELDHLGHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTEFQLT
PFEMLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERQLRPVPQKQRTLHEKIL
EEIKQERRLRPVGAQHLGGRGFGSLPCILNACSGDIKSTSCINLSVTDTGSGSQRPRPRVLLKAPTLAEM
EEMNTSEEEESPCGEVALKRDRSFSEHDLAQLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQ
GFCPVSGQSQPLPSSALPSSLSSVDGPEAASPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERF
LQDKELFSSLKRGKICCCCRAKFPLFSWPPTCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGFESLQRV
PTTKATPTLRRDAFQSLQGPKWRSVEEEFPHIYAHGCVLKDVCSDCTSFVADVVCSSRKSVDVLNATPRR
SRQTQSLYIPNTRTLNFQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-MYC/DDK
Predicted MW 80.2 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_758491
Locus ID 234857
UniProt ID Q8K1S6
Cytogenetics 8 E1
RefSeq Size 2399
RefSeq ORF 2154
Synonyms BC026502; Spir-2; Spir2
Summary Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703, PubMed:21983562). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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