DDX17 (NM_030881) Human Recombinant Protein

CAT#: TP323076

Recombinant protein of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), transcript variant 2, 20 µg

Product Images

Coomassie blue staining of purified DDX17 protein (Cat# TP323076). The protein was produced from HEK293T cells transfected with DDX17 cDNA clone (Cat# [RC223076]) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC223076 representing NM_030881
  Red=Cloning site Green=Tags(s)
  
  MQLVDHRGGG GGGGKGGRSR YRTTSSANNP NLMYQDECDR RLRGVKDGGR RDSASYRDRS
  ETDRAGYANG SGYGSPNSAF GAQAGQYTYG QGTYGAAAYG TSSYTAQEYG AGTYGASSTT
  STGRSSQSSS QQFSGIGRSG QQPQPLMSQQ FAQPPGATNM IGYMGQTAYQ YPPPPPPPPP
  SRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDK
• Tag: C-Myc/DDK
• Predicted MW: 19 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_112020
• Locus ID: 10521
• UniProt ID: Q92841
• Cytogenetics: 22q13.1
• Refseq Size: 4092
• Refseq ORF: 552
• Synonyms: DKFZp761H2016; P72; RH70
• Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]