AHNAK (NM_024060) Human Recombinant Protein

CAT#: TP315337M

Recombinant protein of human AHNAK nucleoprotein (AHNAK), transcript variant 2, 100 µg

Product Images

Coomassie blue staining of purified AHNAK protein (Cat# TP315337). The protein was produced from HEK293T cells transfected with AHNAK cDNA clone (Cat# RC215337) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC215337 representing NM_024060
  Red=Cloning site Green=Tags(s)
  
  MEKEETTRELLLPNWQGSGSHGLTIAQRDDGVFVQEVTQNSPAARTGVVKEGDQIVGATIYFDNLQSGEV
  TQLLNTMGHHTVGLKLHRKGDRSPEPGQTWTREVFSSCSSEVFLNTPQPSALECKDQNKQKEASSQAGAV
  SVSTPNAGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 15.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_076965
• Locus ID: 79026
• UniProt ID: Q09666, Q9BVU3
• Cytogenetics: 11q12.3
• Refseq Size: 1108
• Refseq ORF: 447
• Synonyms: AHNAKRS; PM227
• Summary: The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene. [provided by RefSeq, Oct 2016]
• Protein Families: Protease