Adiponectin (ADIPOQ) (NM_004797) Human Recombinant Protein

CAT#: TP315161M

Recombinant protein of human adiponectin, C1Q and collagen domain containing (ADIPOQ), 100 µg

Product Images

Coomassie blue staining of purified ADIPOQ protein (Cat# TP315161). The protein was produced from HEK293T cells transfected with ADIPOQ cDNA clone (Cat# RC215161) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC215161 representing NM_004797
  Red=Cloning site Green=Tags(s)
  
  MLLLGAVLLLLALPGHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGD
  PGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQ
  NHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQ
  VWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 24.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_004788
• Locus ID: 9370
• UniProt ID: Q15848, A8K660
• Cytogenetics: 3q27.3
• Refseq Size: 4592
• Refseq ORF: 732
• Synonyms: ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
• Summary: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus