Brk(PTK6) (NM_005975) Human Recombinant Protein

CAT#: TP307766

Recombinant protein of human PTK6 protein tyrosine kinase 6 (PTK6)

Bulk Request/Custom Protein Development

 Product Datasheet for 'TP307766'

USD 438.00

USD 730.00

In Stock

    • 20 ug

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Product Data
Description Recombinant protein of human PTK6 protein tyrosine kinase 6 (PTK6)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC207766. Click on the TrueORF clone link to view cDNA and protein sequences.
Predicted MW 51.7 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity PTK6 activity verified in a biochemical assay: PTK6 (protein tyrosine kinase 6) (TP307766) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. PTK6 is a cytoplasmic nonreceptor protein tyrosine kinase which may function as an intracellularsignal transducer in epithelial tissues. Varying concentrations of PTK6 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Reference Data
RefSeq NP_005966
Locus ID 5753
Refseq Size 2519
Cytogenetics 20q13.33
Refseq ORF 1353
Synonyms BRK; BRK
Summary Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins. [UniProtKB/Swiss-Prot Function]
Protein Families
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