C6orf57 (SDHAF4) (NM_145267) Human Recombinant Protein

CAT#: TP305542

Recombinant protein of human chromosome 6 open reading frame 57 (C6orf57), 20 µg

Product Images

Coomassie blue staining of purified SDHAF4 protein (Cat# TP305542). The protein was produced from HEK293T cells transfected with SDHAF4 cDNA clone (Cat# RC205542) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC205542 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPSRLPWLLSWVSATAWRAARSPLLCHSLRKTSSSQGGKSELVKQSLKKPMLPEGRFDAPEDSHLEKEP
  LEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCIDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 12 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_660310
• Locus ID: 135154
• UniProt ID: Q5VUM1
• Cytogenetics: 6q13
• Refseq Size: 526
• Refseq ORF: 324
• Synonyms: C6orf57; Sdh8
• Summary: Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol (PubMed:24954416). Binds to the flavoprotein subunit SDHA in its FAD-bound form, blocking the generation of excess reactive oxigen species (ROS) and facilitating its assembly with the iron-sulfur protein subunit SDHB into the SDH catalytic dimer (By similarity).[UniProtKB/Swiss-Prot Function]
• Protein Families: Secreted Protein