C6orf57 (SDHAF4) (NM_145267) Human Mass Spec Standard

CAT#: PH305542

C6orf57 MS Standard C13 and N15-labeled recombinant protein (NP_660310)

Product Images

Coomassie blue staining of purified SDHAF4 protein (Cat# TP305542). The protein was produced from HEK293T cells transfected with SDHAF4 cDNA clone (Cat# RC205542) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205542
• Predicted MW: 12.2 kDa
• Protein Sequence:
  Sequence Link

  >RC205542 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPSRLPWLLSWVSATAWRAARSPLLCHSLRKTSSSQGGKSELVKQSLKKPMLPEGRFDAPEDSHLEKEP
  LEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCIDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_660310
• RefSeq Size: 526
• RefSeq ORF: 324
• Synonyms: C6orf57; Sdh8
• Locus ID: 135154
• UniProt ID: Q5VUM1
• Cytogenetics: 6q13
• Summary: Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol (PubMed:24954416). Binds to the flavoprotein subunit SDHA in its FAD-bound form, blocking the generation of excess reactive oxigen species (ROS) and facilitating its assembly with the iron-sulfur protein subunit SDHB into the SDH catalytic dimer (By similarity).[UniProtKB/Swiss-Prot Function]
• Protein Families: Secreted Protein