Protein G Streptococcus Protein

CAT#: RA101HRP

Protein G streptococcus protein, 1 mg

Specifications

Product Data

• Species: Streptococcus
• Concentration: lot specific
• Purity: This product was prepared from Chromatographically pure Protein G.
• Conjugation: HRP
• Buffer: State: Lyophilized purified Ig fraction
  Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: Horseradish Peroxidase (HRP)
  Presentation Label: HRP
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent)
• Preparation: Lyophilized purified Ig fraction
• Applications: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
  Recommended Dilutions:
  ELISA: 1/20,000-1/200,000.
  Western blot: 1/10,000-1/40,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).
• Protein Description: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A.
• Note: Caution: Do Not Add Sodium Azide.
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for several weeks at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.