Protein G Streptococcus Protein
Specifications
| Product Data | |
| Species | Streptococcus |
|---|---|
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G. Protein G Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A. |
| Conjugation | HRP |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized purified Ig fraction |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A. |
| Note | Protein G Peroxidase has been tested by ELISA and dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein G Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| UniProt ID | P19909 |
| Synonyms | ProG, Streptococcus G protein, Protein G HRP, peroxidase, Horseradish peroxidase |
| Summary | Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). |
| Protein Families | 000-1/200, 000-1/40, 000-1/5, 000. Immunohistochemistry: 1/1, 000. Western blot: 1/10, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays. Recommended Dilutions: ELISA: 1/20, Suitable for Immunoblotting (Western or dot blot) |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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