Protein G Streptococcus Protein
Specifications
| Product Data | |
| Species | Streptococcus |
|---|---|
| Concentration | 2.0 mg/mL by UV absorbance at 280 nm |
| Purity | Protein G Fluorescein Conjugate was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein G. No reaction was observed against anti-Protein A. |
| Conjugation | FITC |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL |
| Preparation | Lyophilized purified Ig fraction. |
| Recommended Dilution | FLISA,Flow Cytometry,IF Microscopy |
| Protein Description | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein G. No reaction was observed against anti-Protein A. |
| Note | Protein G Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| UniProt ID | P19909 |
| Synonyms | ProG, Streptococcus G protein, Protein G FITC |
| Summary | Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Protein G is also coupled with reporter molecules such as a fluorescein, peroxidase, biotin and other detection reagents. |
| Protein Families | 000-1/5, 000-1/50, 000., 000. IF Microscopy: 1/1, fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. This product is designed for Immunofluorescence Microscopy, utilizing various commercial platforms. FLISA: 1/10 |
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