Protein A (Peroxidase conjugated) Staphylococcus aureus Protein

CAT#: RA100HRP

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Protein A (Peroxidase conjugated) s. aureus protein, 2 mg


Product Datasheet for 'RA100HRP'


USD 320.00


Availability*
5 Days

Size
    • 2 mg


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Specifications

Product Data
Description Protein A (Peroxidase conjugated) s. aureus protein, 2 mg
Species Staphylococcus aureus
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Purity This product was prepared from chromatographically pure Protein A.
Buffer State: Lyophilized purified protein
Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative: 0.01% (w/v) Gentamicin Sulfate
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Presentation Label: HRP
Reconstitution Restore with 1.0 ml of deionized water (or equivalent).
Preparation Lyophilized purified protein
Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/200,000.
Western Blot: 1/10,000-1/40,000.
Immunohistochemistry: 1/1,000-1/5,000. 
Note: Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).
Protein Description Horseradish Peroxidase (HRP) conjugated Protein A.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. 
No reaction was observed against anti-Protein G
Note Molecular Weight:  (protein A): 40,000 daltons.

Caution: Do Not Add Sodium Azide.
Storage Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Stability Shelf life: one year from despatch.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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