Streptavidin Protein

CAT#: BA662

Streptavidin protein, 5 mg

Specifications

Product Data

• Protein Source: S. avidinii
• Predicted MW: 53 kDa
• Purity: >95% pure (SDS-PAGE)
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free)
  Buffer System: PBS
  Preservative: 1% Mannitol
  Stabilizer: 1% Dextran
• Bioactivity: Biological: One unit will bind 1 µg of D-Biotin.
  Specific: 17 U/mg Streptavidin
• Reconstitution: Restore with distilled water or saline.
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation: Lyophilized Streptavidin from Streptomyces avidinii (Essentially salt free)
• Applications: Can be used for biotinylated antibody detection and quantification by ELISA, blotting and immunohistology; as a ligand for immobilization on activated matrices.
• Protein Description: Purified Streptavidin prepared by Affinity Chromatography
• Note: pI: 5
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.