GFAP Bovine Protein

CAT#: BA1011S

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GFAP bovine protein, 0.1 mg


Product Datasheet for 'BA1011S'


USD 280.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description GFAP bovine protein, 0.1 mg
Species Bovine
Protein Source Spinal Cord
Predicted MW 52 kDa
Concentration 1 mg/ml
Purity >98% (determined by SDS gelelectrophoresis).
Buffer Presentation State: Purified
State: Lyophilized
Buffer System: Final Solution contains 10mM Sodium Phosphate buffer pH 7.5, 6M Urea, 2mM DTT, 1 mM EDTA, 10mM Methylammonium Chloride
Reconstitution Restore with distilled water.
BA1011S: 80 µl (final volume 100 µl).
BA1011 : 200 µl (final volume 250 µl).
Preparation Lyophilized
Applications Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
Protein Description Bovine Glial Filament Protein (GFP)
Note Isoelectric Point: pI 5.4
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_776490
Locus ID 281189
Summary glial fibrillary acidic protein

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