GFAP Bovine Protein
|Description||GFAP bovine protein, 0.1 mg|
|Protein Source||Spinal Cord|
|Predicted MW||52 kDa|
|Purity||>98% (determined by SDS gelelectrophoresis).|
|Buffer||Presentation State: Purified
Buffer System: Final Solution contains 10mM Sodium Phosphate buffer pH 7.5, 6M Urea, 2mM DTT, 1 mM EDTA, 10mM Methylammonium Chloride
|Reconstitution||Restore with distilled water.
BA1011S: 80 µl (final volume 100 µl).
BA1011 : 200 µl (final volume 250 µl).
|Applications||Protein standard in 1D and 2D SDS gelelectrophoresis.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
|Protein Description||Bovine Glial Filament Protein (GFP)|
|Note||Isoelectric Point: pI 5.4|
|Storage||Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at -20°C.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Summary||glial fibrillary acidic protein|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.