Leukemia inhibitory factor Mouse Protein

CAT#: AR31198PU-S

Leukemia inhibitory factor mouse recombinant protein, 5 µg

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
• Predicted MW: 19.9 kDa
• Purity: >98% by SDS-PAGE and HPLC analysis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized (0.2µ Sterile filtered) purified protein with no Additives
• Bioactivity: Biological: Murine LIF is fully biologically active when compared to standards.
  The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.
• Endotoxin: < 0.1 ng per µg (1EU/µg)
• Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge the vial bevor opening.
  Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation: Lyophilized (0.2µ Sterile filtered) purified protein with no Additives
• Protein Description: Recombinant Mouse LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001034626
• Locus ID: 16878
• UniProt ID: P09056, F8WH42
• Cytogenetics: 11 2.94 cM
• Summary: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.[UniProtKB/Swiss-Prot Function]