Thioredoxin / TRX1 (1-105, His-tag) Human Protein

CAT#: AR09430PU-N

Thioredoxin / TRX1 (1-105, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
• Tag: His-tag
• Predicted MW: 13.9 kDa
• Concentration: lot specific
• Purity: >95% by SDS – PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: PBS, pH 7.4
• Bioactivity: Biological: Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
  Activity Assay
  1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
  2. Add 4 ug of recombinant His-TXN into reaction cocktail and mix immediately.
  3. Incubate at 25°C for 20 minutes.
  4. Record the increase in A650nm for 15 minutes.
• Preparation: Liquid purified protein
• Protein Description: Recombinant TXN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001231867
• Locus ID: 7295
• UniProt ID: P10599
• Cytogenetics: 9q31.3
• Synonyms: TRDX; TRX; TRX1; Trx80
• Summary: The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Protein Families: Druggable Genome