Glyoxalase I / GLO1 (1-184) Human Protein

CAT#: AR09139PU-N

Glyoxalase I / GLO1 (1-184) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
• Predicted MW: 20.7 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
• Bioactivity: Biological: >1.7 units/ml (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of protein.
  Activity Assay
  Final assay concentrations: In 1.5ml reaction mix, the final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin, 0.033% methylglyoxal (w/v) and recombinant glyoxalase I (0.5ug, 1ug, 2ug).

  1. Prepare 1.45ml reaction buffer without recombinant protein into suitable container (for each protein concentration).
  2. Equilibrate protein-free reaction buffer to 25C and monitor at A240nm until the value is constant, using a spectrophotometer.
  3. Dilute various concentrations of recombinant glyoxalase I (0.5ug, 1ug, 2ug) in 50ul of 10mM potassium phosphate buffer with 0.1% (w/v) BSA, pH 7.4.
  4. Add 50ul of recombinant-glyoxalase I to 1.45ml reaction buffer.
  5. Immediately mix by inversion and record the increase at A240nm for 5 minutes.

• Preparation: Liquid purified protein
• Protein Description: Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_006699
• Locus ID: 2739
• UniProt ID: Q04760, X5DNM4
• Cytogenetics: 6p21.2
• Synonyms: GLOD1; GLYI; HEL-S-74
• Summary: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]
• Protein Pathways: Pyruvate metabolism