Interleukin-2 / IL2 (C145S)

CAT#: AR09046PU-L

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This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


 Product Datasheet for 'AR09046PU-L'

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USD 1,070.00


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2 Weeks

Size
    • 500 ug


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Product Data
Description This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Species Human
Expression Host E. coli
Predicted MW 15.4 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS PAGE
Buffer PBS, pH 7.4
Bioactivity Measured in a cell proliferation assay using CTLL2
mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/ml.
Activity Assay
1.\tCell line: CTLL2 (mouse cytotoxic T cell)
2.\tMaintenance Condition: 10% FBS RPMI 1640 with hIL2
3.\tAssay medium: 10% FBS RPMI 1640 without hIL2
4.\tCell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.\tStarvation : 24hr, Assay medium
6.\tIncubation Time : 24hr (after sample treatment)
7.\tConcentration Range: 0.039 ng/ml - 40 ng/ml
8.\tDetection method : MTT assay
Preparation Liquid purified protein
Reference Data
RefSeq NP_000577
Locus ID 3558
Cytogenetics 4q27
Synonyms IL-2; lymphokine; TCGF
Summary The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus

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68 Mouse Clones