Adenylate kinase 2 / AK2 (1-239, His-tag) Human Protein

CAT#: AR09002PU-N

Adenylate kinase 2 / AK2 (1-239, His-tag) human recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI
• Tag: His-tag
• Predicted MW: 28.6 kDa
• Concentration: lot specific
• Purity: >95% by SDS-PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris pH 7.5, 5 mM DTT, 20% glycerol
• Bioactivity: Specific: > 1.5 units/ml
  One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25°C.
• Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare a 1.4 ml assay buffer (Assay buffer: 58 mM glycylglycine, 2.0 mM adenosine 5'-diphosphate, 2.3 mM beta-nicotinamide adenine dinucleotide phosphate, 10 mM magnesium chloride, 10 mM glucose, 2 unit hexokinase, 1 unit glucose-6-phosphate dehydrogenase, 0.003% (w/v) BSA, pH 7.5.)
  - G-6-PDH/Hex (sigma,Cat.No. H-8629)
  2. Add 50 ul of recombinant Adenylate kinase isoenzyme 2 protein with various concentrations (0.5 ug, 1 ug) and read the increase in A340nm for 5 minutes.
• Protein Description: Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001186128
• Locus ID: 204
• UniProt ID: P54819, P54819-5
• Cytogenetics: 1p35.1
• Synonyms: ADK2
• Summary: Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism