Adenylate kinase 2 / AK2 (1-239, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 28.6 kDa (259aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM DTT, 20% glycerol |
| Bioactivity | Specific activity is > 25unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Enzyme Activity |
| Protein Description | Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_001616.1 |
| Locus ID | 204 |
| UniProt ID | P54819 |
| Cytogenetics | 1p35.1 |
| Synonyms | Adenylate kinase 2, Mitochondrial Adenylate kinase 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, ADK2 |
| Summary | Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.provided by RefSeq, Nov 2010 |
| Protein Categories | Immune system diseases, Intracellular Proteins |
| Protein Families | 0.003% (w/v) BSA, 1 ug) and read the increase in A340nm for 5 minutes., 1 unit glucose-6-phosphate dehydrogenase, 10 mM glucose, 10 mM magnesium chloride, 2 unit hexokinase, 2.0 mM adenosine 5'-diphosphate, 2.3 mM beta-nicotinamide adenine dinucleotide phosphate, Cat.No. H-8629) 2. Add 50 ul of recombinant Adenylate kinase isoenzyme 2 protein with various concentrations (0.5 ug, pH 7.5.) - G-6-PDH/Hex (sigma, Protocol: Activity Assay 1. Prepare a 1.4 ml assay buffer (Assay buffer: 58 mM glycylglycine |
| Protein Pathways | Metabolic pathways, Purine metabolism |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Discovery of genetic variants of the kinases that activate tenofovir among individuals in the United States, Thailand, and South Africa: HPTN067
Figueroa, Tillotson, Li et al
PLoS ONE (2018) 13 (4), e0195764 DOI: 10.1371/journal.pone.0195764
Figueroa, Tillotson, Li et al
PLoS ONE (2018) 13 (4), e0195764 DOI: 10.1371/journal.pone.0195764
Showing 1-1 of 1 papers.
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