MAFG (NM_002359) Human Mass Spec Standard

CAT#: PH321486

MAFG MS Standard C13 and N15-labeled recombinant protein (NP_002350)

Product Images

Coomassie blue staining of purified MAFG protein (Cat# TP321486). The protein was produced from HEK293T cells transfected with MAFG cDNA clone (Cat# RC221486) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC221486
• Predicted MW: 17.7 kDa
• Protein Sequence:
  Sequence Link

  >RC221486 representing NM_002359
  Red=Cloning site Green=Tags(s)
  
  MTTPNKGNKALKVKREPGENGTSLTDEELVTMSVRELNQHLRGLSKEEIVQLKQRRRTLKNRGYAASCRV
  KRVTQKEELEKQKAELQQEVEKLASENASMKLELDALRSKYEALQTFARTVARSPVAPARGPLAAGLGPL
  VPGKVAATSVITIVKSKTDARS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002350
• RefSeq Size: 5043
• RefSeq ORF: 486
• Synonyms: hMAF
• Locus ID: 4097
• UniProt ID: O15525, A0A024R8X1
• Cytogenetics: 17q25.3
• Summary: Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]
• Protein Families: Druggable Genome, Transcription Factors