NFE4 (NM_001085386) Human Mass Spec Standard

SKU
PH320641
NFE4 MS Standard C13 and N15-labeled recombinant protein (NP_001078855)
  $3,360.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC220641
Predicted MW 20.33 kDa
Protein Sequence
Protein Sequence
>RC220641 protein sequence
Red=Cloning site Green=Tags(s)

LPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQLLLCACTSVTDLT
YWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPRGHAPSAEDPTGSWTVSGPCKDHPH
PFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_001078855
RefSeq Size 967
RefSeq ORF 537
Synonyms NF-E4
Locus ID 58160
Cytogenetics 7q22.1
Summary The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. provided by RefSeq, Jul 2008
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Other "NFE4" proteins (3)
SKU Description Size Price
LC421283 Lyophilized NFE4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY421283 Transient overexpression lysate of transcription factor NF-E4 (NF-E4) (as WB positive control) 100 ug
$436.00
TP320641 Recombinant protein of human transcription factor NF-E4 (NF-E4), 20 µg 20 ug
$867.00

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