Pepsin (PGA4) (NM_001079808) Human Mass Spec Standard

CAT#: PH319574

PGA4 MS Standard C13 and N15-labeled recombinant protein (NP_001073276)

Product Images

Coomassie blue staining of purified PGA4 protein (Cat# TP319574). The protein was produced from HEK293T cells transfected with PGA4 cDNA clone (Cat# RC219574) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC219574
• Predicted MW: 42 kDa
• Protein Sequence:
  Sequence Link

  >RC219574 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLEN
  YLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTG
  SMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQ
  DLFSVYLSADDKSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGETIACAEGCQAIVDTGT
  SLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQG
  MNVPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001073276
• RefSeq Size: 1413
• RefSeq ORF: 1164
• Locus ID: 643847
• UniProt ID: P00790, P0DJD7, A0A1S5UZ02, B7Z719
• Cytogenetics: 11q12.2
• Summary: This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
• Protein Families: Druggable Genome