Sterol carrier protein 2 (SCP2) (NM_001007100) Human Mass Spec Standard

CAT#: PH318251

SCP2 MS Standard C13 and N15-labeled recombinant protein (NP_001007101)

Product Images

Coomassie blue staining of purified SCP2 protein (Cat# TP318251). The protein was produced from HEK293T cells transfected with SCP2 cDNA clone (Cat# RC218251) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC218251
• Predicted MW: 15.08 kDa
• Protein Sequence:
  Sequence Link

  >RC218251 representing NM_001007100
  Red=Cloning site Green=Tags(s)
  
  MGFPEAARTHQIEAVPTSSASDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVD
  VKNGKGSVLPNSDKKADCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNLQLQPGNAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001007101
• RefSeq Size: 1438
• RefSeq ORF: 420
• Synonyms: NLTP; NSL-TP; SCOX; SCP-2; SCP-CHI; SCP-X; SCPX
• Locus ID: 6342
• UniProt ID: P22307, Q59HG9
• Cytogenetics: 1p32.3
• Summary: This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms.[provided by RefSeq, Aug 2010]
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis