Transglutaminase 5 (TGM5) (NM_201631) Human Mass Spec Standard

CAT#: PH315126

TGM5 MS Standard C13 and N15-labeled recombinant protein (NP_963925)

Product Images

Coomassie blue staining of purified TGM5 protein (Cat# TP315126). The protein was produced from HEK293T cells transfected with TGM5 cDNA clone (Cat# RC215126) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC215126
• Predicted MW: 80.8 kDa
• Protein Sequence:
  Sequence Link

  >RC215126 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQGLEVALTDLQSSRNNVRHHTEEITVDHLLVRRGQAFNLTLYFRNRSFQPGLDNIIFVVETGPLPDLA
  LGTRAVFSLARHHSPSPWIAWLETNGATSTEVSLCAPPKAAVGRYLLKIHIDSFQGSVTAYQLGEFILLF
  NPWCPEDAVYLDSEPQRQEYVMNDYGFIYQGSKNWIRPCPWNYGQFEDKIIDICLKLLDKSLHFQTDPAT
  DCALRGSPVYVSRVVCAMINSNDDNGVLNGNWSENYTDGANPAEWTGSVAILKQWNATGCQPVRYGQCWV
  FAAVMCTVMRCLGIPTRVITNFDSGHDTDGNLIIDEYYDNTGRILGNKKKDTIWNFHVWNECWMARKDLP
  PAYGGWQVLDATPQEMSNGVYCCGPASVRAIKEGEVDLNYDTPFVFSMVNADCMSWLVQGGKEQKLHQDT
  SSVGNFISTKSIQSDERDDITENYKYEEGSLQERQVFLKALQKLKARSFHGSQRGAELQPSRPTSLSQDS
  PRSLHTPSLRPSDVVQVSLKFKLLDPPNMGQDICFVLLALNMSSQFKDLKVNLSAQSLLHDGSPLSPFWQ
  DTAFITLSPKEAKTYPCKISYSQYSQYLSTDKLIRISALGEEKSSPEKILVNKIITLSYPSITINVLGAA
  VVNQPLSIQVIFSNPLSEQVEDCVLTVEGSGLFKKQQKVFLGVLKPQHQASIILETVPFKSGQRQIQANM
  RSNKFKDIKGYRNVYVDFAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_963925
• RefSeq Size: 2777
• RefSeq ORF: 2160
• Synonyms: PSS2; TGASE5; TGASEX; TGM6; TGMX; TGX
• Locus ID: 9333
• UniProt ID: O43548, B4DPS8
• Cytogenetics: 15q15.2
• Summary: This gene encodes a member of the transglutaminase family. The encoded protein catalyzes formation of protein cross-links between glutamine and lysine residues, often resulting in stabilization of protein assemblies. This reaction is calcium dependent. Mutations in this gene have been associated with acral peeling skin syndrome. [provided by RefSeq, Oct 2009]
• Protein Families: Druggable Genome