GTPBP10 (NM_033107) Human Mass Spec Standard

CAT#: PH310451

GTPBP10 MS Standard C13 and N15-labeled recombinant protein (NP_149098)

Product Images

Coomassie blue staining of purified GTPBP10 protein (Cat# TP310451). The protein was produced from HEK293T cells transfected with GTPBP10 cDNA clone (Cat# RC210451) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210451
• Predicted MW: 42.9 kDa
• Protein Sequence:
  Sequence Link

  >RC210451 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHCSCVLFRKYGNFIDKLRLFTRGGSGGMGYPRLGGEGGKGGDVWVVAQNRMTLKQLKDRYPGKRFVAG
  VGANSKISALKGSKGKDWEIPVPVGISVTDENGKIIGELNKENDRILVAQGGLGGKLLTNFLPLKGQKRI
  IHLDLKLIADVGLVGFPNAGKSSLLSCVSHAKPAIADYAFTTLKPELGKIMYSDFKQISVADLPGLIEGA
  HMNKGMGHKFLKHIERTRQLLFVVDISGFQLSSHTQYRTAFETIILLTKELELYKEELQTKPALLAVNKM
  DLPDAQDKFHELMSQLQNPKDFLHLFEKNMIPERTVEFQHIIPISAVTGEGIEELKNCIRKSLDEQANQE
  NDALHKKQLLNLWISDTMSSTEPPSKHAVTTSKMDII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_149098
• RefSeq Size: 7550
• RefSeq ORF: 1161
• Synonyms: ObgH2; UG0751c10
• Locus ID: 85865
• UniProt ID: A4D1E9
• Cytogenetics: 7q21.13
• Summary: Small G proteins, such as GTPBP10, act as molecular switches that play crucial roles in the regulation of fundamental cellular processes such as protein synthesis, nuclear transport, membrane trafficking, and signal transduction (Hirano et al., 2006 [PubMed 17054726]).[supplied by OMIM, Mar 2008]