PDE6H (NM_006205) Human Mass Spec Standard

CAT#: PH310215

PDE6H MS Standard C13 and N15-labeled recombinant protein (NP_006196)

Product Images

Coomassie blue staining of purified PDE6H protein (Cat# TP310215). The protein was produced from HEK293T cells transfected with PDE6H cDNA clone (Cat# RC210215) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210215
• Predicted MW: 9.1 kDa
• Protein Sequence:
  Sequence Link

  >RC210215 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDNTTLPAPASNQGPTTPRKGPPKFKQRQTRQFKSKPPKKGVKGFGDDIPGMEGLGTDITVICPWEAFS
  HLELHELAQFGII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006196
• RefSeq Size: 763
• RefSeq ORF: 249
• Synonyms: ACHM6; RCD3
• Locus ID: 5149
• UniProt ID: Q13956
• Cytogenetics: 12p12.3
• Summary: This gene encodes the inhibitory (or gamma) subunit of the cone-specific cGMP phosphodiesterase, which is a tetramer composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma). It is specifically expressed in the retina, and is involved in the transmission and amplification of the visual signal. Mutations in this gene are associated with retinal cone dystrophy type 3A (RCD3A). [provided by RefSeq, Mar 2010]
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism