AADACL1 (NCEH1) (NM_020792) Human Mass Spec Standard

CAT#: PH306188

NCEH1 MS Standard C13 and N15-labeled recombinant protein (NP_065843)

Product Images

Coomassie blue staining of purified NCEH1 protein (Cat# TP306188). The protein was produced from HEK293T cells transfected with NCEH1 cDNA clone (Cat# RC206188) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC206188
• Predicted MW: 49.1 kDa
• Protein Sequence:
  Sequence Link

  >RC206188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSCRGQKVAGGLRVVSPFPLCQPAGEPSRGKMRSSCVLLTALVALAAYYVYIPLPGSVSDPWKLMLLDA
  TFRGAQQVSNLIHYLGLSHHLLALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRS
  VVYIHGGGWALASAKIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFLKPEVLQKY
  MVDPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNTPILPRYVM
  VKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNARIVQELPQ
  LLDARSAPLIADQAVLQLLPKTYILTCEHDVLRDDGIMYAKRLESAGVEVTLDHFEDGFHGCMIFTSWPT
  NFSVGIRTRNSYIKWLDQNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_065843
• RefSeq Size: 4294
• RefSeq ORF: 1320
• Synonyms: AADACL1; NCEH
• Locus ID: 57552
• UniProt ID: Q6PIU2, A0A0R4J2G3
• Cytogenetics: 3q26.31
• Summary: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.[UniProtKB/Swiss-Prot Function]
• Protein Families: Transmembrane