Seryl tRNA synthetase (SARS) (NM_006513) Human Mass Spec Standard

CAT#: PH303665

SARS MS Standard C13 and N15-labeled recombinant protein (NP_006504)

Product Images

Coomassie blue staining of purified SARS protein (Cat# TP303665). The protein was produced from HEK293T cells transfected with SARS cDNA clone (Cat# RC203665) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203665
• Predicted MW: 58.7 kDa
• Protein Sequence:
  Sequence Link

  >RC203665 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKK
  KEPVGDDESVPENVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGNLLH
  PSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQY
  ALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALH
  RDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEE
  FYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKV
  EFVHMLNATMCATTCTICAILENYQTEKGITVPEKLKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEG
  SKKKAAARDVTLENRLQNMEVTDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006504
• RefSeq Size: 1956
• RefSeq ORF: 1542
• Synonyms: NEDMAS; SARS; SERRS; SERS
• Locus ID: 6301
• UniProt ID: P49591, Q0VGA5
• Cytogenetics: 1p13.3
• Summary: This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010]
• Protein Pathways: Aminoacyl-tRNA biosynthesis