Seryl tRNA synthetase (SARS) (NM_006513) Human Tagged ORF Clone

CAT#: RC203665

SARS (Myc-DDK-tagged)-Human seryl-tRNA synthetase (SARS), transcript variant 1

Product Images

Circular map for RC203665

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  Western blot validation of overexpression lysate (Cat# LY416590) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203665 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SARS protein (Cat# TP303665). The protein was produced from HEK293T cells transfected with SARS cDNA clone (Cat# RC203665) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Seryl tRNA synthetase
• Synonyms: NEDMAS; SARS; SERRS; SERS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203665 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGGATCTGGATTTGTTTCGGGTGGATAAAGGAGGGGACCCAGCCCTCATCCGAGAGACGCAGG
  AGAAGCGCTTCAAGGACCCGGGACTAGTGGACCAGCTGGTGAAGGCAGACAGCGAGTGGCGACGATGTAG
  ATTTCGGGCAGACAACTTGAACAAGCTGAAGAACCTATGCAGCAAGACAATCGGAGAGAAAATGAAGAAA
  AAAGAGCCAGTGGGAGATGATGAGTCTGTCCCAGAGAATGTGCTGAGTTTCGATGACCTTACTGCAGACG
  CTTTAGCTAACCTGAAAGTCTCACAAATCAAAAAAGTCCGACTCCTCATTGATGAAGCCATCCTGAAGTG
  TGACGCGGAGCGGATAAAGTTGGAAGCAGAGCGGTTTGAGAACCTCCGAGAGATTGGGAACCTTCTGCAC
  CCTTCTGTACCCATCAGTAACGATGAGGATGTGGACAACAAAGTAGAGAGGATTTGGGGTGATTGTACAG
  TCAGGAAGAAGTACTCTCATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGGGCCGT
  GGTGGCTGGGAGTCGAGGGTACTTCTTGAAGGGGGTCCTGGTGTTCCTGGAACAGGCTCTCATCCAGTAT
  GCCCTTCGCACCTTGGGAAGTCGGGGCTACATTCCCATTTATACCCCCTTTTTCATGAGGAAGGAGGTCA
  TGCAGGAGGTGGCACAGCTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGTGAAAA
  GTCTGATGACAACTCCTATGATGAGAAGTACCTGATTGCCACCTCAGAGCAGCCCATTGCTGCCCTGCAC
  CGGGATGAGTGGCTCCGGCCGGAGGACCTGCCCATCAAGTATGCTGGCCTGTCTACCTGCTTCCGTCAGG
  AGGTGGGCTCCCATGGCCGTGACACCCGTGGCATCTTCCGAGTCCATCAGTTTGAGAAGATTGAACAGTT
  TGTGTACTCATCACCCCATGACAACAAGTCATGGGAGATGTTTGAAGAGATGATTACCACCGCAGAGGAG
  TTCTACCAGTCCCTGGGGATTCCTTACCACATTGTGAATATTGTCTCAGGTTCTTTGAATCATGCTGCCA
  GTAAGAAGCTTGACCTGGAGGCCTGGTTTCCGGGCTCAGGAGCCTTCCGTGAGTTGGTCTCCTGTTCTAA
  TTGCACGGATTACCAGGCTCGCCGGCTTCGAATCCGATATGGGCAAACCAAGAAGATGATGGACAAGGTG
  GAGTTTGTCCATATGCTCAATGCTACCATGTGCGCCACTACCTGTACCATCTGCGCCATCCTGGAGAACT
  ACCAGACAGAGAAGGGCATCACTGTGCCTGAGAAATTGAAGGAGTTCATGCCGCCAGGACTGCAAGAACT
  GATCCCCTTTGTGAAGCCTGCGCCCATTGAGCAGGAGCCATCAAAGAAGCAGAAGAAGCAACATGAGGGC
  AGCAAAAAGAAAGCAGCAGCAAGAGACGTCACCCTAGAAAACAGGCTGCAGAACATGGAGGTCACCGATG
  CT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203665 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKK
  KEPVGDDESVPENVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGNLLH
  PSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQY
  ALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALH
  RDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEE
  FYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKV
  EFVHMLNATMCATTCTICAILENYQTEKGITVPEKLKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEG
  SKKKAAARDVTLENRLQNMEVTDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006513
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006513.4
• RefSeq Size: 1956 bp
• RefSeq ORF: 1545 bp
• Locus ID: 6301
• UniProt ID: P49591
• Cytogenetics: 1p13.3
• Domains: tRNA-synt_2b, Seryl_tRNA_N
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 58.7 kDa
• Gene Summary: This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010]