Lin28 (LIN28A) (NM_024674) Human Mass Spec Standard

CAT#: PH303397

LIN28A MS Standard C13 and N15-labeled recombinant protein (NP_078950)

Product Images

Coomassie blue staining of purified LIN28A protein (Cat# TP303397). The protein was produced from HEK293T cells transfected with LIN28A cDNA clone (Cat# RC203397) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203397
• Predicted MW: 22.7 kDa
• Protein Sequence:
  Sequence Link

  >RC203397 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPV
  DVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCY
  NCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_078950
• RefSeq Size: 4014
• RefSeq ORF: 627
• Synonyms: CSDD1; LIN-28; lin-28A; LIN28; ZCCHC1
• Locus ID: 79727
• UniProt ID: Q9H9Z2
• Cytogenetics: 1p36.11
• Summary: This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015]
• Protein Families: Transcription Factors