Proteasome beta 1 (PSMB1) (NM_002793) Human Mass Spec Standard

CAT#: PH301798

PSMB1 MS Standard C13 and N15-labeled recombinant protein (NP_002784)

Product Images

Coomassie blue staining of purified PSMB1 protein (Cat# TP301798). The protein was produced from HEK293T cells transfected with PSMB1 cDNA clone (Cat# RC201798) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201798
• Predicted MW: 26.5 kDa
• Protein Sequence:
  Sequence Link

  >RC201798 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSSTAMYSAAGRDLGMEPHRAAGPLQLRFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPK
  CYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGG
  LDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISA
  AERDVYTGDALRICIVTKEGIREETVSLRKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002784
• RefSeq Size: 938
• RefSeq ORF: 723
• Synonyms: HC5; PMSB1; PSC5
• Locus ID: 5689
• UniProt ID: P20618, A0A140VK45
• Cytogenetics: 6q27
• Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq, Jul 2008]
• Protein Families: Protease
• Protein Pathways: Proteasome