CDC42EP3 (NM_006449) Human Mass Spec Standard

CAT#: PH301069

CDC42EP3 MS Standard C13 and N15-labeled recombinant protein (NP_006440)

Product Images

Coomassie blue staining of purified CDC42EP3 protein (Cat# TP301069). The protein was produced from HEK293T cells transfected with CDC42EP3 cDNA clone (Cat# RC201069) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201069
• Predicted MW: 27.7 kDa
• Protein Sequence:
  Sequence Link

  >RC201069 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAKTPIYLKAANNKKGKKFKLRDILSPDMISPPLGDFRHTIHIGKEGQHDVFGDISFLQGNYELLPGNQ
  EKAHLGQFPGHNEFFRANSTSDSVFTETPSPVLKNAISLPTIGGSQALMLPLLSPVTFNSKQESFGPAKL
  PRLSCEPVMEEKAQEKSSLLENGTVHQGDTSWGSSGSASQSSQGRDSHSSSLSEQYPDWPAEDMFDHPTP
  CELIKGKTKSEESLSDLTGSLLSLQLDLGPSLLDEVLNVMDKNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006440
• RefSeq Size: 5715
• RefSeq ORF: 762
• Synonyms: BORG2; CEP3; UB1
• Locus ID: 10602
• UniProt ID: Q9UKI2
• Cytogenetics: 2p22.2
• Summary: This gene encodes a member of a small family of guanosine triphosphate (GTP) metabolizing proteins that contain a CRIB (Cdc42, Rac interactive binding) domain. Members of this family of proteins act as effectors of CDC42 function. The encoded protein is involved in actin cytoskeleton re-organization during cell shape changes, including pseudopodia formation. A pseudogene of this gene is found on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]