S6K1 (RPS6KB1) CytoSection

CAT#: TS417324P5

Transient overexpression of RPS6KB1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

Immunohistology staining on S6K1 overexpressed cytosection TS417324P5 with mouse anti DDK clone OTI11C3 C/N TA180144 at 1:400 dilution 4C O/N. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC217324, 5 micron sections, 40x magnification

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC217324
• Tag: C-MYC/DDK
• Detection Antibodies: DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags (TA592569)
• Target Detection Antibodies: S6K1 (RPS6KB1) Mouse Monoclonal Antibody [Clone ID: OTI6B2] (TA502929)
• Accession Number: NM_003161, NP_003152
• Other Names: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_003152
• Locus ID: 6198
• Cytogenetics: 17q23.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway