IL10 CytoSection

CAT#: TS416785P5

Transient overexpression of IL10 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

Immunohistology staining on IL10 overexpressed cytosection TS416785P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC216785, 5 micron sections, 40x magnification

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC216785
• Tag: C-MYC/DDK
• Detection Antibodies: DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags (TA592569)
• Target Detection Antibodies: IL10 Mouse Monoclonal Antibody [Clone ID: OTI9H3] (TA813305)
• Accession Number: NM_000572, NP_000563
• Other Names: CSIF; GVHDS; IL-10; IL10A; TGIF
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_000563
• Locus ID: 3586
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway