Isocitrate dehydrogenase (IDH1) CytoSection

CAT#: TS410582P5

Transient overexpression of IDH1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

Immunohistology staining on IDH1 overexpressed cytosection TS410582 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC210582, 5 micron sections, 40x magnification.

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  Immunohistology staining on IDH1 overexpressed cytosection TS410582 with mouse anti IDH1 clone OTI3G9 C/N TA800406 at 1:300 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC210582, 5 micron sections, 40x magnification.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC210582
• Tag: C-MYC/DDK
• Detection Antibodies: DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags (TA592569)
• Target Detection Antibodies: Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: OTI2H9] (TA500610)
• Accession Number: NM_005896, NP_005887
• Other Names: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_005887
• Locus ID: 3417
• Cytogenetics: 2q34
• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways