Human MIER1 (NM_001077704) AAV Particle

CAT#: RC223062A1V

AAV ORF Particles, serotype AAV-2, MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 6, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: MIER1
• Synonyms: ER1; MI-ER1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223062 representing NM_001077704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATC
  CATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGG
  AGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTTCTC
  AGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAG
  AAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAA
  TATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCT
  TCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAG
  AAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCAT
  GTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAAAATGATGAT
  CAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAA
  GAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTT
  ATATGAATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCA
  GCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATG
  GAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTA
  TTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAGAAGAAATAT
  AATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTC
  GAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGT
  AAGCACTGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGCATACTCCAAATGCTTCTTCCAGTTCAT
  TTTTCAGCCATCAGTTCAAGAGCCAATGCCTTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223062 representing NM_001077704
  Red=Cloning site Green=Tags(s)
  
  MAEPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELL
  SLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVA
  SQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGICRYKENEKVYENDD
  QLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKA
  AREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKY
  NLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGILQMLLPVH
  FSAISSRANAFLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001077704
• ORF Size: 1299 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001077704.1
• RefSeq Size: 1648 bp
• RefSeq ORF: 1302 bp
• Locus ID: 57708
• UniProt ID: Q8N108
• Cytogenetics: 1p31.3
• MW: 48.9 kDa