Mouse Idh1 (NM_010497) AAV Particle

CAT#: MR217871A1V

AAV ORF Particles, serotype AAV-2, Idh1 (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 1 (NADP+), soluble (Idh1), transcript variant 2, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Idh1
• Synonyms: AI314845; AI788952; E030024J03Rik; Id-1; Idh-1; Idpc
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217871 representing NM_010497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGAAAAATCCAAGGAGGTTCTGTGGTGGAGATGCAAGGAGATGAAATGACACGAATCATTTGGG
  AATTGATTAAGGAAAAACTTATTCTTCCCTATGTGGAACTGGATCTGCATAGCTATGATTTAGGCATAGA
  GAATCGTGATGCCACCAATGACCAGGTCACCAAAGATGCTGCAGAGGCTTTAAAGAAATACAACGTGGGC
  GTCAAGTGTGCTACCATCACCCCCGATGAGAAGAGGGTTGAAGAATTCAAGTTGAAACAAATGTGGAAAT
  CCCCAAATGGCACCATCCGAAACATTCTGGGTGGCACTGTCTTCAGGGAAGCTATTATCTGCAAAAATAT
  CCCCCGGCTAGTGACAGGCTGGGTAAAACCCATCATCATTGGCCGACATGCATATGGGGACCAATACAGA
  GCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAAAGATGGAACTCAGA
  AGGTGACATACATGGTACATGACTTTGAAGAAGGTGGTGGTGTTGCCATGGGCATGTACAACCAGGATAA
  GTCAATTGAAGACTTTGCACACAGTTCCTTCCAAATGGCTCTGTCCAAGGGCTGGCCTTTGTATCTCAGC
  ACCAAGAACACTATTCTGAAGAAGTATGATGGGCGTTTCAAAGACATCTTCCAGGAGATCTATGACAAGA
  AATACAAGTCCCAGTTTGAAGCTCAGAAGATCTGCTATGAACACAGGCTCATAGATGACATGGTGGCCCA
  AGCTATGAAGTCCGAGGGAGGCTTCATCTGGGCCTGTAAGAATTACGATGGGGATGTGCAGTCAGACTCA
  GTCGCCCAAGGTTATGGCTCCCTTGGCATGATGACCAGTGTGCTGATTTGTCCAGATGGTAAGACGGTAG
  AAGCAGAGGCTGCCCATGGCACTGTCACACGTCACTACCGCATGTACCAGAAAGGGCAAGAGACGTCCAC
  CAACCCCATTGCTTCCATTTTTGCCTGGTCCCGAGGGTTAGCCCACAGAGCAAAGCTTGATAACAATACT
  GAGCTCAGCTTCTTCGCAAAGGCTTTGGAAGACGTCTGCATTGAGACCATTGAGGCTGGCTTTATGACTA
  AGGACTTGGCTGCTTGCATTAAAGGCTTACCCAATGTACAACGTTCTGACTACTTGAATACATTTGAGTT
  TATGGACAAACTTGGAGAAAACTTGAAGGCCAAATTAGCTCAGGCCAAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217871 representing NM_010497
  Red=Cloning site Green=Tags(s)
  
  MSRKIQGGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEALKKYNVG
  VKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYR
  ATDFVVPGPGKVEITYTPKDGTQKVTYMVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLS
  TKNTILKKYDGRFKDIFQEIYDKKYKSQFEAQKICYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS
  VAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNT
  ELSFFAKALEDVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKAKLAQAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_010497
• ORF Size: 1242 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_010497.3, NP_034627.3
• RefSeq Size: 2270 bp
• RefSeq ORF: 1245 bp
• Locus ID: 15926
• UniProt ID: O88844
• Cytogenetics: 1 32.91 cM
• MW: 46.7 kDa