Human M-CSF (CSF1) ELISA KIT (1 x 96 wells)

CAT#: EA102898

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human M-CSF. 96wells/kit, with removable strips.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human M-CSF
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Specificity: This kit is used for quantitative detection of Human M-CSF
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti-human CSF1 antibody | 1
  • Lyophilized recombinant human CSF1 standard | 10ng/tubex2
  • Biotinylated anti-human OPG antibody | 130ul (50x)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 400ul (30x)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20ml
  • 
    
• Background: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
• Gene Symbol: CSF1
• Gene ID: 1435