Human ANGPTL2 ELISA KIT (1 x 96 wells)

CAT#: EA102716

For quantitative detection of human ANGPTL2 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human ANGPTL2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312 pg/ml - 20,000 pg/ml
• Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of Human ANGPTL2
• Sensitivity: <50pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti-human ANGPTL2 antibody | 1
  • Lyophilized recombinant human ANGPTL2 standard | 20 ng/tube x 2
  • Biotinylated anti-human ANGPTL2 antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
  • 
    
• Background: Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
• Gene Symbol: ANGPTL2
• Gene ID: 23452