Mouse Tff1 ELISA KIT (1 x 96 wells)

CAT#: EA102444

For Quantitative Detection of mouse TFF1 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Tff1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Specificity: This kit is used for quantitative detection of Mouse Tff1
• Sensitivity: <10pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components: 96-well plate precoated with anti- mouse TFF1 antibody | 1
  Lyophilized recombinant mouse TFF1 standard | 10ng/tube×2
  Biotinylated anti- mouse TFF1 antibody | 100μl(dilution 1:100)
  Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100)
  Sample diluent buffer | 30ml
  Antibody diluent buffer | 12ml
  ABC diluent buffer | 12ml
  TMB color developing agent | 10ml
  TMB stop solution | 10ml
  Adhesive cover | 4
• Background: TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
• Gene Symbol: Tff1
• Gene ID: 21784