Human Testosterone ELISA kit

CAT#: EA102176

Human Testosterone ELISA kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Streptavidin based ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0-18ng/ml
• Sample Type: Serum
• Sample Volume: 50µl/well
• Specificity: This kit is used for quantitative detection for human Testosterone
• Sensitivity: 0.2ng/ml
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Streptavidin Microwell coated plate: 12x8x1
  • 2. Standard: 6 vials (ready to use): 0.5ml
  • 3. Enzyme Conjugate (20X): 1 bottle: 0.7ml
  • 4. Anti-Testosterone Biotin Reagent: 1 bottle (Ready to use): 7ml
  • 5. Assay Diluent: 1 bottle (Ready to use): 12ml
  • 6. TMB Substrate: 1 bottle (ready to use): 12ml
  • 7. Wash Buffer (20X): 1 bottle: 25ml
  • 8. Stop Solution: 1 bottle (Ready to use): 12ml
• Background: Testosterone (17β -hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the β position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. The Testosterone EIA kits are designed for the measurement of total Testosterone in human serum.