Mouse Angiopoietin-2 ELISA Kit

CAT#: EA100593

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Mouse Angiopoietin-2 ELISA Kit

USD 534.00

3 Weeks

    • 1 x 96 wells

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Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Angiopoietin-2
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Angiopoietin-2
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 96-well plate precoated with anti- mouse Angiopoietin-2 antibody 1
  • Lyophilized recombinant mouse Angiopoietin-2 standard 10ng/tubex2
  • Biotinylated anti- mouse Angiopoietin-2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Angiopoietin-2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. Human and mouse ANGPT2 are 85% identical in amino acid sequence and approximately 60% identical to their ANGPT1 homologs. Transgenic overexpression of ANGPT2 in mice disrupted blood vessel formation in the mouse embryo. By FISH and radiation hybrid analysis, the human ANGPT2 gene was mapped to 8p23, and in a region of syntenic homology on mouse chromosome 8, where the Angpt2 gene was mapped. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis .
Gene Symbol Angpt2
Gene ID 11601
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