Human IL-8 ELISA Kit (96-well)
|Description||Human IL-8 ELISA Kit (96-well)|
|Type||Solid Phase Sandwich ELISA|
|Size||1 x 96 wells|
|Format||96-well strip plate|
|Assay Type||Solid Phase Sandwich ELISA|
|Assay Length||3 hours|
|Sample Type||Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)|
|Sample Volume||20 uL|
|Specificity||Natural and recombinant Human IL-8 Ligand|
|Interference||No significant interference observed with available related molecules.|
Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them.
The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form.
IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors.
Representative standard curve for IL-8 ELISA. IL-8 was diluted in serial two-fold steps in Sample Diluent.
|The use of this Assay Kits has been cited in the following citations:|
Proinflammatory Effects of Calprotectin in Graves' Orbitopathy
,Kim, JW;Ko, J;Kim, J;Yoon, JS;,
Ocul. Immunol. Inflamm. 2018
,PubMed ID 30452877
|Assay Kit Resources|