F(ab')2 Swine IgG (H&L) Antibody Peroxidase Conjugated

CAT#: TA398258

F(ab')2 Swine IgG (H&L) Antibody Peroxidase Conjugated

Product Images

ELISA Results of Rabbit F(ab')2 Anti-Swine IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Swine IgG (p/n 014-0102). The workiing dilution is 105000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS buffer (p/n MB-066), and TMB substrate (p/n TMBE-1000).

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:500 - 1:2,500
  ELISA: 1:10,000 - 1:100,000
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Swine IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG and Swine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: F(ab')2 Anti-Swine IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.