Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated
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USD 200.00
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000 |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Rat IgG whole molecule |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rat IgG and Rat Serum. |
Formulation | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | Alkaline Phosphatase |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Note | Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
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