Mouse IgG (H&L) Antibody Rhodamine Conjugated

CAT#: TA397805

Mouse IgG (H&L) Antibody Rhodamine Conjugated

Product Images

Western Blot of Rhodamine Conjugated Goat anti-Mouse IgG Secondary Antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.

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  Western Blot of Rhodamine Conjugated Goat anti-Mouse IgG Secondary Antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: IF: 1:1,000 - 1:5,000
  FC: 1:500 - 1:2,500
  FLISA: 1:10,000 - 1:50,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 2.0 mg/mL - lot specific
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is available in a variety of formats. Anti IgG secondary antibody conjugated is suitable for ELISA, Immunohistochemistry western blotting as well as other peroxidase antibody based assays.