Mouse IgG2b Antibody Rhodamine Conjugated Pre-adsorbed

CAT#: TA397801

Mouse IgG2b Antibody Rhodamine Conjugated Pre-adsorbed

Product Images

Dot Blot of Goat anti-Mouse IgG2b Antibody Rhodamine Conjugated Pre-absorbed. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2b Antibody Rhodamine Conjugated Pre-absorbed at 1:1,000 overnight at 4°C. Block: MB-070 for 1 HR at RT.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:1,000 - 1:5,000
  IF: 1:1,000 - 1:5,000
  ELISA: 1:2,000 - 1:10,000
  FLISA: 1:20,000 - 1:100,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG2b heavy chain
• Specificity: Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Anti-Mouse IgG2b Rhodamine antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2b antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats.