Human IgG4 Mouse Monoclonal Antibody [Clone ID: MRQ-44]

CAT#: AM33493PU-N

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Specifications

Product Data

• Clone Name: MRQ-44
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500.
  Preparation and Pretreatment:
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
  Positive Control: Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Human IgG4
• Specificity: Human IgG4.
  Cellular Localization: Cytoplasmic.
• Formulation: Tris Buffer, pH 7.3-7.7
  State: Purified
  State: Liquid purified Tissue Culture Supernatant.
  Stabilizer: 1% BSA
  Preservative: < 0.1% Sodium Azide
• Form: Purified
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!