Bovine IgG (light chain) Mouse Monoclonal Antibody [Clone ID: IVA285-1]

CAT#: AM03077PU-N

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Product Images

Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).

Specifications

Product Data

• Clone Name: IVA285-1
• Applications: FC, IHC, WB
• Recommended Dilution: Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
  SDS-PAGE (12% separating gel).
  Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
  Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Bovine, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fraction of Bovine immunoglobulin
• Specificity: The antibody IVA285-1 reacts with Bovine IgG (light chains). IgG light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Form: Purified
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!